List of posters

Poster viewing I (Monday, 25 August)

Theme I – New Technologies
Theme II – Predictive Toxicology (sessions II-1 – II-10)

Poster viewing II (Tuesday, 26 August)

Theme II – Predictive Toxicology (sessions II-11 – II-17)
Theme III – 3Rs in Academia & Education
Theme IV – Communication, Dissemination and Data Sharing
Theme V – Efficacy and Safety Testing of Drugs and Biologicals

Poster viewing III (Wednesday, 27 August)

Theme VI – Human Relevance
Theme VII – Ethics
Theme VIII – Refinement and Welfare
Theme IX – Global Cooperation, Regulatory Acceptance and Standardization
Theme X – Additional sessions


Theme I – New Technologies

Session I-1: Virtual tissue models

Performance standards for human epidermis model Keraskin-VM for skin irritation alternatives
Y. Heo, K.-U. Yeo, W.-H. Jang, S.-S. An, K.-M. Lim, S.-H. Seok, J.-H. Che, Y.-S. Yun

Application of zebra fish and Neoderm®-ME, a new 3D pigmented skin model for the evaluation of anti-melanogenic effects of hexapeptoids; PAL-10 and PAL-12
M. Lee, K. E. Joo, K.-M. Lim

Session I-2: High throughput screening (HTS) models

A new approach to the automatic segmentation and evaluation of pigmentation lesion by using active contour model and speeded up robust features
M. Farshad, S. Mardani Samani, Z. Karimi, M. Yazdi

A novel in vitro model of spinal cord injury: validation and treatment strategies
S. D. Boomkamp, M. D. Houslay, S. C. Barnett

Physiological Oxygen Concentrations In A Sandwich Culture On Gas-Permeable Membranes Remarkably Enhance Rat Hepatocytes Functions And Genes Expression
W. Xiao, H. Matsui, M. Shinohara, K. Komori, T. Osada, Y. Sakai

A feasibility study into the biodistribution of test compounds in zebrafish embryos
J. McCormack, V. van der Weijden, T. Maarschalkerweerd, C. Snel, A. Menke, E. Hansen, F. Van Goethem, N. Mesens, A. Wolterbeek

Cellular Ellman's method adaptation using SH-SY5Y is a possibility for neurochemical multiparametric evaluation
R. Bachinski, L. Hummel, A. Linhares, A. Meyer, J. M. Granjeiro, G. G. Alves

A data analysis pipeline for Tox21 Phase II quantitative high throughput screening assays and its application to toxicity profiling of flame retardants
J.-H. Hsieh, A. Sedykh, M. Behl, R. Huang, M. Xia, R. Tice

Automation-compatible EST assay
S. Stroebel, D. Fluri, W. Moritz, J. M. Kelm

Evaluation Method in Bhas 42 Cell Transformation Assay Selecting Transformed Foci Using Hydrogen Peroxide, H2O2
K. Yamakage, D. Muramatsu, S. Arai, N. Endo, K. Sasaki

Using Skin-PAMPA for transdermal patch testing
B. Sinko, G. Vizseralek, K. Tsinman

Session I-3: Tissue on a chip / Human-on-a-chip

µ3DVasc: a novel microfluidic bioreactor setup for the reconstruction of vascularized 3D tissues in vitro
D. Ivannikov, I. Hebeiß, P. Nikolov, S. Giselbrecht, U. Schepers

Serial linkage of biochips toward an organ-on-chip approach
W. Wirths, J. Wiest, B. Wolf

Cell-based rapid and quantitative toxicity and efficacy monitoring in consecutive before and after the compound metabolism within liver for two ginger compounds at physiological concentration
T. Ona, J. Shibata

A multi-organ-chip co-culture of human liver equivalents and neurospheres for long-term substance testing
E.-M. Materne, A. Ramme, A. Lorenz, A. P. Terrasso, C. Brito, P. M. Alves, R. Lauster, U. Marx

Emulating the human vasculature in a multi-organ-chip platform
T. Hasenberg, E.-M. Materne, K. Schimek, S. Bauer, S. Brincker, A. Lorenz, R. Lauster, U. Marx

3D-printing: a new dimension for the 3R's
M van Zijverden, S Deleu

Long-term culture of dermal units in multi-organ-chips
K. Schimek, G. Lindner, B. Atac, A. Wanzek, D. Reissmann, I. Wagner, R. Lauster, U. Marx

Development of a 3-dimesional tissue with vascular networks in vitro
Y. Liu, S. Sakai, M. Taya

Synthesis and Characterization of Tunable Agarose-Gelatin Cryogel for Hepatotoxicity Evaluation
P. Balaji, S. Shanmugaapriya, M. A. Akbarsha

Neurotoxicity in vitro: assessment of the predictivity of neuronal networks coped to microelectrode arrays for identification of neurotoxicants
T. Ramirez, T. Weisschu, H. A. Huener, R. Landsiedel, B. van Ravenzwaay

Automated long-term operation of multi-organ-chips
L. Kloke, A. Jänicke, A. Ramme, F. Sonntag, C. Polk, F. Schmieder, M. Bürger, V. Artyushenko, U. Marx, R. Lauster

Substance exposure to skin and liver co-cultures in a Multi-Organ-Chip
I. Wagner, J. Hübner, T. Lade, R. Horland, A. Schepky, R. Lauster, J. Kuehnl, U. Marx

A new human liver 3D culture in microfluidic platform as a potential tool for toxicity studies
M. Jang, P. Neuzil, S.J. Trietsch, A. Manz

A Multi-Organ-Chip-based dynamic sensitization assay comprising human epidermis and primary dendritic cell co-culture
J. Hübner, I. Wagner, A. Lorenz, T. Lade, S. Gerlach, A. Schepky, J. Kühnl, R. Horland, R. Lauster, U. Marx

How new technologies are promising to fulfil the Reduction and Replacement 3Rs objectives
M. Lewis, J. M. Wilkinson, E. Kingsley

Kidney toxicity assessment and ADMET testing in a two-circuit microfluidic device
C. Drewell, A. Ramme, L. Aschauer, P. Jennings, F. Schmieder, F. Sonntag, R. Lauster, U. Marx

Microtissues meet microfluidic: A multi-parallel body on a chip concept
J.-Y. Kim, D. Fluri, J. G. Hengstler, R. Marchan, J. M. Kelm, A. Hierlemann, O. Frey

Session I-4: Novel 3D models

The Assessment of a Caco-2/CCD-18co Co-culture Model of the Small Intestine Cultured on Poly (Ethylene Terephthalate) Nanofibre Scaffolds
J. Patient, K Harris, B Abrahamsson , C Tannergren, A Ghaemaghammi, P Williams, C Roberts, L White, FRAJ Rose

New BEST - biomaterials enhanced simulation test
M. Gasik

An in vitro ocular test system for a detailed quantification of the cellular damage in the corneal epithelium and stroma
M. Bartok, D. Gabel, M. Zorn-Kruppa, M. Engelke

The initial depth of injury in 3-dimensional tissue models for the prediction of the eye-irritation potential of chemicals
M. Zorn-Kruppa, M. Bartok, M. Engelke, K. R. Mewes, K. Daton, K. Reisinger, J. M. Brandner

A 3D Culture System for Investigating Inflammatory Responses in Human Lung
S. S. Htwe, H. Harrington, J. W. Haycock, J. Aylott, A. M. Ghaemmaghami

A new 3D human airway tissue model for in vitro lung cancer research (OncoCilAir™)
C. Mas, S. Huang, L. Wiszniewski, S. Constant

A novel 3d-culture system activating hepatic function of HepG2 cells utilizing a collagen vitrigel membrane chamber and its application to liver metabolism and toxicity test
A. Oshikata-Miyazaki, S. Ishida, T. Takezawa

Offbeat to mainstream: high-throughput, user-friendly three-dimensional cell culture models of brain tumors
D. Ivanov, C. Alexander, D. Walker, T. Parker, P. Gellert, M. Ashford, M. Garnett

Human and rat neuronal microtissues for toxicity testing
J. M. Kelm, D. Fluri, R. Marchan, J. G. Hengstler

Human organotypic nasal epithelial tissue culture as an in vitro model to evaluate effects of cigarette smoke
M. Cabanski, C. Mathis, S. Frentzel, D. Kuehn, S. Majeed, C. Merg , M. Geertz , E. Guedj, R. Dulize, D. Peric, K. Trivedi, A. Benyagoub, Y. Xiang, F. Martin, P. Leroy, N. V. Ivanov, M. C. Peitsch, J. Hoeng

Pre-validation of the Hen´s Egg Test for Micronucleus-Induction (HET-MN): Final evaluation of data
K. Reisinger, D. Fieblinger, A. Heppenheimer, J. Kreutz, M. Liebsch, K. Maul, R. Pirow, A. Poth, P. Strauch, T. Wolf

3D Skin Comet assay: Status quo of the ongoing validation
K. Reisinger M. Bartel, V. Blatz, J. Brinkmann, T. R. Downs, U. Engels, A. Fischer, F. Henkler, S. Hoffmann, S. Jeschke, C. Krul, M. Liebsch, A. Luch, R. Pirow, A. Reus, M. Schulz, S. Pfuhler

Development of an immune-competent model of human upper airway epithelium
W. Aldajani, F. Salazar, H. Harrington, A. Ghaemmaghami

Effect of ATP suppliers on viability of cryopreserved cells
A. Jamshidzadeh, M. Nematizadeh, H. Niknahad, M, Hosseinaghdaie

Global proteomic analysis of acetaminophen toxicity in 3D human liver microtissues
C. Escher, R. Ossola, P. Gunness, M. Bober, F. Grimm, L. Reiter, Y. Butscheid, J. M. Kelm, S. Messner

Biological assay device of neurologic cells with in situ observable gel culture system composed of ECM-modelized matrix
H. Mizumachi, Y. Harada, H. Ijima

The evaluation of spheroid culture of human hepatocytes for drug induced hepatotoxicity
M. Motoi-Ohtsuji, D. Nagai, M. Kakiki, J. Katagi, N. Matsumoto, S. Kaneda, H. Iwai, T. Nagao, T. Ikeya, E. Ozeki, T. Jomura, S. Tanaka, K. Yahiro, T. Tamura, T. Ueda, K. Naitoh, S. Maruya, K. Wada, T. Ohkura, K. Kusumoto, Y. Inoue, H. Arakawa, T. Ogihara

In vitro 3D scaffold-free osteoarthritis model - multiple application options
A. Lang, J. Neuhaus, D. Barnewitz, I. Ponomarev

Human cell-based functional 3D-angiogenesis test for identification of inhibitors of angiogenesis
T. Toimela, O. Huttala, J-R. Sarkanen, T. Heinonen

Evaluation of human hepatocytes cultured by three-dimensional spheroid systems for drug metabolism
H. Arakawa, T. Ohkura, K. Ohta, T. Nagao, K. Kusumoto, A. Koeda, T. Ueda, T. Jomura, T. Ikeya, E. Ozeki, K. Naitoh, Y. Inoue, N. Takahashi, H. Iwai, T. Ogihara

In vitro human alveolar tissue model for pulmonary drug delivery and toxicology applications
P. J. Hayden, G. R. Jackson, J. Oldach, C. Mankus, S. Ayehunie, M. Klausner, H. Kandarova

Importance of reproducibility demonstration of the bio-engineered tissue models used for in vitro toxicity testing purposes
S. Letasiova, M. Bachelor, H. Kandarova, K. Sebova, P. Kearney, M. Klausner

Reconstructed 3-D human small intestinal tissue to assess drug safety, inflammation, and restitution
S. Ayehunie, Z.Stevens, T.Landry, M. Klausner , P. Hayden

LUHMES 3D neuronal model for (developmental) neurotoxicity testing
L. Smirnova, G. Harris, H. Hogberg, D. Pamies, L. Zhao, R. Bachinski, J. Bressler, J. Delp, M. Leist, T. Hartung

A human iPSC-derived 3D model for the assessment of gene/environment interactions during neurodevelopment
G. Harris, D. Pamies, J. Bressler, M. Palma-Lima, L. Smirnova, K. Block, K. M. Christian, C. Zhang, T. Hartung, H. Hogberg

Using human organotypic tissue slices in cancer research
F. Merz, S. Kallendrusch, M. Gerlach, N. Schopow, J. Koerfer, S. Hoebel, A. Aigner, F. Gaunitz, I. Bechmann

The importance of normalization and standardization of cytotoxicity assays for 3D cell models
R. Bachinski, T. Hartung, J. M. Granjeiro, G. G. Alves, H. Hogberg

BIOMEMS for accurate in vivo - in vitro correlation
S.Angeloni Suter

Development of a New Reconstituted Human Oral Mucosal Model to Assess the Oral Irritation Testing
S. Lee, H. Jung, S. Kim

Session I-5: Bioreactors

Rapid fabrication of engineered liver tissue using novel fibroblast system
Y. Sakai, M. Koike, H. Hasegawa, K. Yamanouchi, A. Soyama, M. Hidaka, M. Takatsuki, T. Kuroki, T. Okano, S. Eguchi

Human Fibroblast Remodelling When Cultured In 3D And Under Flow
H. Harrington, S. Ahmed, S.S.Htwe, P.Cato, F. Rose, M.Wilkinson, J. Aylott, A. M. Ghaemmaghami

Engineering hepatic tissues with perfusable vascular structures
T. Osaki , N. Teshima, T. Kageyama, J. Enomoto, J. Fukuda

Protection from Cell Death in Multicellular Spheroids
W. Motoyama, S. Aoki, N. Kojima

Development of a perfusion chamber for the cultivation of 3D skin constructs
K. Struever, F. Gorreja, W. Friess, S. Küchler

2D and 3D human liver cell bioreactors for long-term repeated-dose toxicity testing as a basis for computer-aided prediction
E. Heinzle, F. Noor, S. Klein, M.Ingelman-Sundberg, A. van Dorsselar, C. Chesné, P. Peters, J. Bucher, J. Hengstler, D. Drasdo

The need of innovative technologies for new 3D relevant in-vitro models and the answer of Ivtech
T. Sbrana, G. Mattei, S. Giusti, A. Ahluwalia

Improving in vitro tests of orthopedic implant materials
M. Giazzon, P. Schneider, M. Favre, A. Meister, M. Liley

Bioengineering approaches for the development of novel 3D in vitro models for pre-clinical applications
S. P. Rebelo, D. Simão, C. Correia, A. Terrasso, C. Pinto, R. Costa, M. Serra, C. Brito, P. M. Alves

Use of dynamic 3D primary hepatocyte culture to understand the effects of constitutive androstane receptor activators in primary rat and human hepatocytes
R. Maclennan, A. Bennett, R. Currie

Dose metric considerations of cationic and anionic surfactants in in vitro cytotoxicity assays
N. I. Kramer, S. T. J. Droge, J. J. H. Haftka, E. Opsahl, A. Zwartsen, F. A. Groothuis, B. Nicol, B. J. Blaauboer

Session I-7: Monitoring (telemetry)

Dual pressure telemetry in rats; measuring interventricular asynchrony in experimental pulmonary hypertension
I. Schalij, M. A. de Raaf, W. J. van der Laarse, H. J. Bogaard, M. L. Handoko, A. Vonk Noordegraaf, K. Kramer

Enhanced monitoring of disease studies involving laboratory mice using radiofrequency identification technology
C. A. Richardson

Correlation of infrared thermography vs. rectal body temperature measurements in baboons (papio spp.)
R. Sullivan, N. Schlabritz-Loutsevitch, T. Mandrell

Development of the mCM – mobile circulatory module – for ex-vivo physiological lung tissue for breathing simulation
M. Forjan, M. Frohner


Theme II – Predictive Toxicology

Session II-1: Pathways approaches in toxicology

In vitro skin sensitization testing strategy - in-house post-validation with “real-life” compounds
K. Guth, S. Kolle, N. Honarvar, B. Wareing, A. Mehling, B. van Ravenzwaay, R. Landsiedel

Developing a Mitochondrial Toxicity Pathway-Based Non-Animal Approach for Chemical Safety Assessment
J. Li, H. Yuan, T. Zhang, J. Guo, S. Peng, D. Suarez-Rodriguez, A. While, P. Carmichael, C. Westmoreland

Development of an alternative testing strategy for the Fish Early Life-Stage (FELS) test: results of the AOP ‘Acetylcholinesterase inhibition leading to motor activity impairment’
S. Verstraelen, P. Berckmans, L. Vergauwen, R. Blust, F. Dardenne, D. Villeneuve, G. Ankley, D. Knapen, H. Witters

An adverse outcome pathway based approach to evaluate novel tobacco products and electronic nicotine delivery devices in vitro
F. Lowe, K. McQuillan, A. Bushby, G. Phillips, D. Breheny

Identification of cornifelin and early gene response-1 as new biomarkers for in vitro eye irritation using a 3D reconstructed human cornea model, MCTT HCE™
S. H. Choi, M. R. Lee, Y. J. Chun, K. M. Lim

Application of Alternative Toxicity Test Methods for Safety Assessment of Active Pharmaceutical Ingredient Intermediates
M. Múčková, M. Bednáriková, J. Lazová, V. Šmahovský, I. Kakalík

C24:1-ceramide may be a novel lipid biomarker for eye irritation in 3D human corneal epithelial model, MCTT HCE™
M. Lee, S. Choi, K.-M. Joo, Y.-J. Chun, K.-M. Lim

Using Adverse Outcome Pathways for Regulatory Applications
C. E. Willett, P. Antczak, L. Burgoon, F. Falciani, S. Gutsell, G. Hodges, A. Kienzler, D. Knapen, M. McBride, E. J Perkins

The Experience of using the alternative in vitro methods for testing and safety assessment of goods for children, detergents, cosmetics, including raw nanomaterials
N. Zavyalov, Е. Skvortsova, O.Guskova

Session II-2: Systems biology

Enlightening the intracellular metabolic phenotype of neuronal cells: prospects of transient isotope tracer experiments
S. Niedenführ, K. Nöh

Chemical grouping and read across: a biology based approach using metabolomics
B. van Ravenzwaay, M. Herold, H. Kamp, E. Fabian, G. Krennrich, R. Looser, W. Mellert, G. A. Montoya, E. Peter, V.A. Prokoudine

A ToxBank Integrated Data Analysis of SEURAT-1 Reference Compounds
B. Hardy, G. Myatt, P. Kohonen, R. Grafström

Session II-3: Computational modeling and chem-informatics

Inhibition screen of OATP1B1, OATP1B1*15 and OATP1B3 combined with computational modeling to predict OATP-mediated drug-drug interactions
J. Venhorst, E. van de Steeg, H.T. Jansen, I.H.G. Nooijen, J. DeGroot, H.M. Wortelboer, M.L.H. Vlaming

Development of a curated database of in vivo estrogenic activity
P. Ceger, N. Kleinstreuer, X. Chang, B. Jones, J. Hamm, L. Rinckel, D. Rotroff, J. Strickland, W. Casey, D. Allen

Prediction of physicochemical properties of environmental chemicals using molecular fingerprints and machine learning methods
Q. Zang, K. Mansouri, D. Allen, W. Casey, R. Judson

Computational approaches for predicting protein targets for chemical toxins based on molecular similarity and inverse docking
Y. Chushak, J. Schlager , D.Mattie , J. Gearhart

QSAR analysis of human skin sensitization potencies
E. Muratov, V. Alves, D. Fourches, J. Strickland, N. Kleinstreuer, C. Andrade, A. Tropsha

Development of in silico (computational) toxicity screening methods
K. Yuta

Issues relating to the availability and standardisation of data for nanomaterials to assist in risk assessment
A.-N. Richarz, R.L. Marchese Robinson , A. Cassano, M.T.D. Cronin

The application of in silico methods for predictions of different toxicological endpoints
M. N. Drwal, P. Banerjee, M. Dunkel, N. Körner, P. Zwicker, M. R. Wettig, B. Haertel, R. Preissner

Applying cheminformatics approaches to support toxicity prediction and safety assessment of chemicals through the development of robust categories
A.-N. Richarz, S.J. Enoch, M.T.D. Cronin, L. Terfloth, C.H. Schwab, C. Yang

Using Adverse Outcome Pathways to support mechanistic in silico modelling: examples with organ level toxicity
M. T. D. Cronin, S.. J. Enoch, J. C. Madden, F. P. Steinmetz, K. P. Przybylak, A.-N. Richarz, C. Yang

Acute oral toxicity modeling accounting for mechanism and toxicological mode of action
D. Nedelcheva, N. Dimitrova, S. Stoeva, A. Detroyer, R. Note, S. Ringeissen, K. Kirilov, S. Dimitrov, O. Mekenyan

A mathematical model for a reproduction-related adverse outcome pathway in fathead minnows (Pimephales promelas)
M. Mayo, K. H. Watanabe, T. Habib, N. Garcia-Reyero, E. J. Perkins

Session II-4: Risk Assessment

Agglomeration influences cytotoxicity of nanovesicle formulations in vitro
M. P. Vinardell, D. R. Nogueira, M. Mitjans

Validation of the Slug Mucosal Irritation test: correlation with clinical data
J. Lenoir, J.P. Remon, E. Adriaens

Animal welfare observations and early toxicologic assessment tools used to improve selection of traditional drug discovery efficacy models
K. R. Sharp, J. E. McDuffie, J. Ma, B. Zimmerman, A. De Smedt, F. Almy, M. Rizzolio, K. Vriens, S. Snook

Use of the zebrafish developmental screen and estimation of internal concentration to assess toxicity
J. Hamm, N. Kleinstreuer, W. Casey, D. Allen, S. Padilla

Leveraging small aquarium fishes to advance understanding of environmentally influenced human disorders and diseases
J. Hamm, W. Casey, M. Behl, D. Hinton, J. Kanungo, S. Kullman, C. Mattingly, A. Planchart, T. Tal, C. Weis

Challenges and opportunities for conducting safety/risk assessments based solely on in vitro data
H. Clewell

In vitro skin corrosion as a pre-validation study of Reconstructed Human Epidermis in house developed
C. Catarino, P. Pennacchi, T. Pedrosa, S. Barros, S. Maria-Engler

Evaluation of immunotoxicity of natural compounds by a combination of functional in-vitro investigation and proteomics
N. Schultze, P. Zwicker, G. Wachlin, M. Drwal, M. Dunkel, M. Lalk, R. Preissner, U. Lindequist, B. Haertel

Integrated test strategy for cosmetics safety and efficacy assessment
S. Cheng, Y. Qin, F. Pan

How to improve the high variability of the in vitro dermal absorption test?
B. Desmedt, P. Courselle, J.O. De Beer, V. Rogiers, E. Deconinck, K. De Paepe

Use of in vitro assays to compare the effects of exposure to smoke and smoke extracts from a conventional cigarette and a reduced toxicant prototype
O. Oke, D. Azzopardi, S. Corke, K. Hewitt, T. Carr, G. Foss-Smith, M. Taylor, K. Patel, F. Lowe

Lentiviral transduction to drive a liver - derived rat epithelial cell line from biliary origin towards hepatocyte-like cells
J. Bolleyn, N. Nair, J. De Kock, M. Chuah, T. VandenDriessche, V. Rogiers, T. Vanhaecke

Optimization of an eye irritation assay for solid materials for hazard identification and labelling of chemicals
Y. Kaluzhny, H. Kandarova, L. d’Argembeau-Thornton , J. De Luca , P. Hayden, A. Hunter, T. Truong, K. Klausner

Risk assessment of consumer products by use of alternative tox methods
M. Deore, A. Patil, D. Bakre, N. Ravishankar

A method proposed to estimate the index of relevance using the statistical imputation approach
M.Endo, T. Omori

Cytotoxic, cytoprotective and genotoxic evaluation of grape pomace extract against hydrogen peroxide insult
L. García-Becerra, M. Mitjans, R. Castro-Ríos, J. Verde-Star, C. Leos-Rivas, C. Rivas-Morales, A. Oranday-Cárdenas, A. Chávez-Montes, P. Vinardell

Isolated intervertebral disc cells as a platform for in vitro toxicology testing
L. Gradisnik, T. Velnar

A review of the REACH legislation from the animal welfare perspective
K. Taylor

Refined animal toxicity testing using unequally sized dose groups and Benchmark Dose analysis
J. Ringblom, F. Kalantari, G. Johanson, M. Öberg

Metabolism simulation and toxicity prediction in the evaluation of food ingredient/contaminant safety
L. Terfloth, K. Arvidson, K. Muldoon-Jacobs, P. Volarath, A. Tarkhov, T. Magdziarz, J. Rathman, D. Hristozov, C. Yang

Comparison of in vitro and in vivo methodologies for eye irritation evaluation of cosmetic formulations
C. A. Brohem, B. B.Swinka, A. C.Weihermann, A. P. M. Canavez, E. L. Rocha, V. M. Di Mambro, O. Kruger, M. Lorencini

Cultured porcine cornea assay using confocal microscopy for high resolution detection and quantification of sub-mild ocular irritation
M. Piehl, M. Carathers, E. Ryan, W. Hahn, D. Cerven, G. DeGeorge

The replacement ocular battery (robatt): an integrated testing strategy for ocular irritation classification
D. Cerven, M. Piehl, M. Carathers, G. DeGeorge

Cytotoxicity and cell adhesion evaluation of chitosan films with herbs actives for burns treatment
B. P. Genesi, P. S. Lopes, C. F. da Silva

Session II-5: Application in decision making and testing strategies

Use of the in vitro Caco-2 assay to predict the oral absorption of aromatic amine permanent hair dyes
C. Obringer, N. Hewitt, J. Manwaring, C. Goebel, H. Rothe

A risk-warning method for reporting adverse events:-An application of Bayesian statistics to the cosmetics industry-
A.Yamashita, T.Omori

Comparison of 3 criteria incorporating variation of index for toxicityof the interleukin 8 luciferase Luc assay (IL-8 Luc assay)
A. Maruya, S. Aiba, Y. Kimura, M. Watanabe, N. Suzuki, K. Saito, Y. Nakajima, Y. Ohmiya, H. Kojima, N. Tanaka

An easy method for estimation of the half-maximal inhibitory concentration using curve fitting appropriate to the concentration-response relationship
A. Mori, N. Suzuki, H. Nagahori, K. Saito, F. Le Coz, M. Kobayashi, A. Maruya, T. Omori

Relationship between IC50 and ID50 value in the Hand1-Luc Embryonic Stem cell Test (Hand1-Luc EST)
M. Kobayashi, N. Suzuki, H. Nagahori, K. Saito, F. Le Coz, A. Mori, A. Maruya, T. Omori

Integrated safety assessment (ISA) of food products
L. van der Geest, H. Bouwmeester, T. Bovee, R. Hoogenboom, E. Kok, A. Peijnenburg

Session II-7: Update from Europe – Alternative testing strategies program

SEURAT-1: Towards Mechanism-based Repeated Dose Systemic Toxicity Testing
M. Schwarz

Session II-8: Meeting new regulatory challenges following the cosmetic ingredients ban – Cosmetics Europe’s research programme on alternatives

In vitro model for assessing impact of mechanical and chemical skin injuries
H. Dabboue, E. Frouin, C. Cardaire, J. Ramos, G. Marti-Mestres

Session II-9: Exposure

Comparison of protocols measuring stratum corneum diffusion/partition coefficients and skin penetration
N. J. Hewitt, R. Cubberley, H. Duplan, J. Eilstein , D. Gerstel, S. Grégoire , C. Jacques-Jamin, M. Klaric, H. Rothe, A. Schepky

Standardized experimental set-up for dermal absorption studies in vitro
K. Guth, E. Fabian, S. Wölk, B. van Ravenzwaay, R. Landsiedel

The Methodology for Evaluating Mucosal Irritation on Human Oral Tissue Model
J. H. Kim, S. A. Cho, J. Y. Choi, J. H. Lee, K. M. Kim, S. An

Development of exposure systems for the in vitro assessment of “heat-not-burn” tobacco and electronic nicotine delivery devices
H. Green, D. Azzopardi

Assessing the skin irritation potential of rinse off personal care products using a reconstructed human epidermis model: exposure time and product dilution impact
F. Lopez, S. Catoire, D. Mora, A. Cano, B. Rodriguez, A. Thelu, A. Josseaume, E. Lambert

AOH Exposure induced oxidative stress in caco-2 cells
C. Fernández-Blanco, G. Font, M. J. Ruiz

Bovine serum albumin binding and toxicity of cationic chemicals in in vitro cell assays
F. A. Groothuis, B. Nicol, B. J. Blaauboer, N. I. Kramer

SDF-1 induced migration of THP-1 cells through a monolayer of human coronary artery endothelial cells promotes differentiation of monocytes to an adhesive phenotype
M. Van der Toorn, S. Frentzel, M. Peitsch, J. Hoeng, H. De Leon

Overcoming the barriers to the uptake of microsampling techniques in regulatory safety studies
S. Jackson

Retrospective analysis on the correlation between cytokine release from a 3D keratinocytes tissue model and clinical human ocular response to mild surfactant systems
R. M. Walters, E. Bruning, L. Fakhrzadeh, C. Mack Correa, E. Atillasoy, K. Martin, J. R. Nash, N. Wilt, G.-E. Costin, H. A. Raabe

Session II-10: Topical toxicity

Digging between photoallergenic or photoirritant behaviour with the RBC-PT
M. Mitjans, M. P. Vinardell

Additional joint research on eye irritation alternative method with human corneal model; LabCyte CORNEA-MODEL24
H. Ikeda, M. Katoh, T. Omori, A. Yamashita, M. Endo, S. Shinoda, S. Hagiwara, T. Kasahara, H. Tahara, S. Nakahara, S. Akiyama, Y. Yoshitake, H. Kojima

Advantage of “Vitrigel-EIT (eye irritancy test) method”: A brief eye irritation test utilizing changes of barrier function after exposing chemicals to a human corneal epithelium model as an indicator
H. Yamaguchi, H. Kojima, T. Takezawa

Assessment of in vitro eye irritation – testing strategy
K. Tegethoff, H.-W. Vohr, G. Stropp

Further refinements of IRR-IS®, an Episkin® based model for quantifying chemical irritation potency
F. Cottrez, E. Boitel, H. Groux

New evaluation method for skin irritation of oil-soluble chemicals with monolayer culture system
N. Imai, Y. Goto, S. Nomura, T. Masunaga

SkinEthic RHE method categorises chemicals between corrosive subcategories and non-corrosive as stated in OECD TG431
N. Alépée, C. Tornier, J. Cotovio

In vitro skin corrosion and irritation assessment of ingredients using EpiSkin methods: Sequential Top-Down and Bottom-Up approaches
N. Alépée, M.H. Grandidier, J. Cotovio

Integrated approach for skin corrosion and skin irritation using in vitro SkinEthic RHE test methods
N. Alépée , M.H. Grandidier, J. Cotovio

Development of a testing strategy to evaluate 24 h-exposure skin irritation using OECD TG439 and new monolayer culture system
S. Nomura, Y. Goto, N. Imai, T. Masunaga

Modification of 3T3 NRU photo-toxicity test conditions for the evaluation of poorly water-soluble substances
A. Toyoda, M. Sugiyama, S. Furihata, K. Nishizumi, H. Itagaki

Cytotoxicity, phototoxicity and genotoxicity evaluation of sucupira oil: a potential new cosmetic ingredient
M. T. Uemura, E. A. Ostroky, M. B. Mathor, N. Andreo-Filho, T. M. Kaneko, D. P. Luco, M. T. F. Bara, J. R. Paula, P. S.Lopes

Integrated CAMVA and BCOP methods to predict eye irritation of cosmetics
Y. Qin, S. Cheng

Comparison of mouse primary hepatocyte spheroids formation on Cell-ableTM plates with various feeder cells
S. Wakuri, R. Saito, K. Sasaki, M. Gondo, N. Endo, H. Sui, K.Yamakage

Introduction of 3D methods for regulatory testing and research in NICS
Zs. Bartos, Z. Forgacs, M. Szivos-Racz, J. Major

Predicting eye stinging using the novel NociOcular assay
L. Krawiec, K. Norman, R. Curren, Forsby, A.

Observations on the use of the bovine corneal opacity and permeability (BCOP) assay to evaluate the eye irritation potential of prototype cosmetic formulations containing salicylic acid, glycolic acid and ethanol
R. Labib, E. Gilberti, G.-E. Costin, D. Gossai, H. Raabe, S. Gettings

Suitability of histopathology as an additional endpoint to the Isolated Chicken Eye test for classification of non-extreme pH detergent and cleaning products
E. Cazelle, C. Eskes, M. Hermann, P. Jones, P. McNamee, M. Prinsen, H. Taylor, M. Wijnands

Tiered approach to the use of alternatives to animal testing for evaluation of eye irritation potential of cosmetic ingredients: hair dye case study
P. McNamee, G. vonBoelcshazy, C. Goebel

The importance of understanding drivers of irritation in vivo for selection of chemicals used in the development and evaluation of in vitro serious eye damage/eye irritation assays: Cosmetics Europe analysis
P. McNamee, N. Alépée, J. Barroso, A De Smedt, B. De Wever, J. Hibatallah, M. Klaric, K. Mewes, M. Millet, U. Pfannenbecker, M. Tailhardat, M. Templier

Skin irritation potential of UV-filters and vitamin A using a full-thickness reconstructed skin model
C. G. Benevenuto, P. C. Pennacchi, A. C. Fruet, S. S. Maria-Engler, L. R. Gaspar

Exploring of new alternative test method for phototoxicity in ophthalmic agent using SIRC cell lines and 3D human reconstituted cornea models
S. Y. Kim, M.-H. Nam, J.-Y. Yun, J. S. Lee, I. Y. Ahn, J. H. Lee, K. H. Choi

Use of ECVAM validated EpiDerm skin corrosion test (EpiDerm SCT) for sub-categorization according to the UN GHS
H. Kandarova, S. Letasiova, T. Milasova, P. Hayden, M. Klausner

Identifying the appropriate protocol for testing surfactants and surfactant-based formulations in the bovine corneal opacity and permeability assay
J. E. Bader, K. G. Norman, H. A. Raabe

Performance of the chorion-allantoic membrane stained by trypan blue (CAM-TBS) of different concentrations of surfactant sodium dodecyl sulfate (SDS) for assessing the eye irritation potential
R. Silva, V. Ribeiro, C. Caldeira, O. Presgrave, I. Delgado

Reconstructed Human Epidermis Test Methods for Skin Corrosion Endpoint: How Can Changes in Prediction Models Improve Final Predictions?
B. Desprez, J. Barroso, C. Griesinger, H. Kandarova, N. Alépée, H. Fuchs

NativeSkin – a standardized ex vivo skin system to predict human skin responses
S. Kurdykowski, A. A. David, C. A. M. Jardet, P. Descargues


Session II-11: Repeated dose toxicity

Pharmacokinetics of serum honokiol with oral administration Magnolia ethanolic extract in Sprague-Dawley rats
Y.-J. Tsai , J.-S. Huang, P.-Y. Tsai, C.-J. Yao, L.-Y. Kuan, H.-L. Chen, T.-Y. Lai, G.-M. Lai, J. Whang-Peng, J.-M. Chang

Do we still need the 2-year rat carcinogenicity study?
C. Krul, R. Woutersen, A. Soffers, D. Kroese, M. Luijten, J. van Benthem

Developing an integrative approach for predicting acute oral toxicity: Lessons learned
R. Note, D. Blanchet, J. Clouzeau, A. Detroyer, D. Duché, L. Le Capitaine, H. Noçairi, G. Ouédraogo, S. Ringeissen, S. Teissier

An integrative approach for the prediction of acute oral toxicity: Past and future
R. Note, H. Noçairi, D. Blanchet, C. Gomes, G. Ouédraogo, S. Teissier, F. Leroy, J. Cotovio

Session II-12: Skin sensitization

Development of a new local lymph node assay that evaluates the elicitation response
K. Yamashita, S. Shinoda, S. Hagiwara, H. Itagaki

An inter-laboratory validation study of IL-8 Luc assay using a stable THP-1-derived IL-8 reporter cell line, THP-G8
Y. Kimura, M. Watanabe, N. Suzuki, K. Saito, Y. Nakajima, Y. Ohmiya, T. Omori, H. Kojima, N. Tanaka, S. Aiba

A dataset on 80 chemicals tested by IL-8 Luc assay
Y. Kimura, C. Fujimura, S. Aiba

Investigation of the use of THP-1 cells and IL-8 release to assess water-insoluble chemicals with the Short Time Exposure test method
K. Narita, H. Kojima, H. Itagaki

EURL ECVAM validation study on skin sensitisation test methods
S. Casati, P. Aeby, A. Angers-Loustau , D. Basketter, T. Cole, S. Hoffmann, J. Richmond

LLNA variability: an essential ingredient for a comprehensive assessment of non-animal skin sensitisation test methods and strategies
S. Hoffmann

Inter-laboratory validation of an in vitro method to classify skin sensitizers
A. J. Clippinger, D. J. Keller, III, J. M. McKim, Jr, H. Witters, A. R. Van Rompay

Predicting skin sensitization using 21st century toxicology
N. Kleinstreuer, J. Strickland, W. Casey

Development of an open-source integrated testing strategy for skin sensitization potency
J. Pirone, M. Smith, N. Kleinstreuer, T. Burns, J. Strickland, Y. Dancik, R. Morris, W. Casey, J. Jaworska, D. Allen

THP-1 assay predicts allergenic potential of chemicals in accordance with ratings given by LLNA and patch tests
C. B. Parise, V. M. Sá-Rocha, J. Z. Moraes

Modelling the skin sensitisation Adverse Outcome Pathway (AOP) for risk assessment
N. Gellatly, R. Cubberley, S. Dhadra, S. Jacquoilleot, G. Maxwell, C. Moore, R. Pendlington, J. Pickles, O. Saib, D. Sheffield, R. Stark, V. Summerfield, C. Mackay

Skin sensitisation EPAA/CEFIC LRI activities progressing non-animal testing strategies
A. Mehling, D. Basketter, J. Crozier, B. Hubesch, I. Manou

Improving dendritic cell-based skin sensitization assays by integrating tolerogenic markers
C. van Helt, S.F. Martin, H. Wenck, A. Schepky, J. Kühnl

Skin sensitization prediction: transferability and reproducibility of the MUSST
C. Piroird, L. Nardelli, M.Aujoulat, A. Hohenstein, N. Pearson, N. Alépée

Techniques for efficient isolation of human Langerhans cells: assessment of potential allergenic compounds
D. P. Luco, D. Steffens, P. S. Lopes, M. T. Uemura, M. B. Mathor

International ring trial of the epidermal equivalent sensitizer potency assay: reproducibility and predictive-capacity
M. A. T. Teunis, S.W. Spiekstra, M. Smits, E. Adriaens, R. Landsiedel, R. Pieters, V. Galbiati, E. Roggen, E. Corsini, S. Gibbs

Evaluation of the direct peptide reactivity assay (DPRA) in identifying epoxy resins with skin sensitizing potential
C. L. Wong, A. L. Lam, S. Ghassabian, M. T. Smith

Photo-safety assessment using multiple in vitro tests
S. Oeda, H. Nishida, M. Hirota, T. Ashikaga, H. Kouzuki, S. Onoue, S. Aiba, Y. Tokura

In-house validation and technology transfer of the GARD assay for the prediction of sensitizing compounds
K. S. Zeller, A. Forreryd, H. Johansson, F. Rydnert, A.-S. Albrekt, C.A.K. Borrebaeck , M. Lindstedt

Development of skin sensitization test method using THP-1 cells cultured on a collagen vitrigel membrane chamber for oily materials
T. Uchino, H. Miyazaki, K. Yamashita, H. Kojima, A. Oshikata-Miyazaki, T. Takezawa, K. Shimizu, T. Akiyama, Y. Ikarashi

Relationship of contact allergen potency and size of the T cell pool and T cell receptor repertoire
P. R. Esser, M. Huber, A. Kolberg, M. Klaric, E. Bachtanian, K. Reichenbach, V. Weber, S. F. Martin

Application of the reconstructed human epidermis model, Keraskin™, for identifying skin sensitizer
W. H. Jang, G. Choi, K. M. Jung, S. A. Cho, S. An, S. H. Seok, Y. Heo, M. Y. Park

Optimisation of the human T Cell Priming Assay for the identification of chemical allergens
V. Mutez, M. Vocanson, P. Esser, M. Klaric, St. Martin, J. F. Nicolas

Performance standards for local lymph node assay: 5-bromo-2-deoxyuridine-flow cytometry method (LLNA: BrdU-FCM) for skin sensitization alternatives
Y. Heo, K-W. Yeo, J-H. Jo, W-H. Jang, M-Y. Park, K-M. Lim, M-S. Jung, E-Y. Jeon

Analysis of in chemico and in silico methods to predict skin sensitization potentials
D. Urbisch, A. Mehling, S. N. Kolle, N. Honarvar, W. teubner, K. Guth, R. Landsiedel

LuSens: a novel reporter gene-cell line to identify skin sensitizers
T. Ramirez, A. Mehling, A. Aumann, T. Eltze, S. Kolle, D. Urbisch, W. Teubner, C. Wruck, R. Landsiedel, B. van Ravenzwaay

Role of ROS and HMGB1 in contact allergen-induced IL-18 production in human keratinocytes
V. Galbiati, A. Papale, C. L. Galli, M. Marinovich, E. Corsini

Variability in data from skin sensitization and reactivity testings
H. Ivanova, A. Detroyer, C. Piroird, C. Gomes, J. Eilstein, I. Popova, C. Kuseva, Y. Karakolev, S. Dimitrov, O. Mekenyan

Epidermal Sensitization Assay (EpiSensA) with reconstructed human epidermis for detecting sensitizing potential of lipophilic chemicals
O. Takenouchi, K. Saito, Y. Nukada, M. Miyazawa, H. Sakaguchi

Estimation of the skin sensitizing potency based on peptide reactivity, dendritic cell activation and read-across using the hair dye 2-methoxymethyl-paraphenylenediamine as example
C. Goebel, J. A. Troutman, J. Hennen, H. Rothe, H.Schlatter, G. F. Gerberick, B. Blömeke

In vitro skin sensitization methods: evaluating the available reference datasets
J. F. Lalko

Development of improved skin sensitization assays based on 3D reconstructed human epidermal equivalents and secreted luciferase reporter activity
P. J. Hayden, M. Miyazawa, P. Lei, C. Mankus, O. Takenouchi, K. Saito, G. R. Jackson, S. T. Andreadis, Y. Ohmiya, H. Sakaguchi

Evaluation of SENS-IS®, an Episkin® based model for identifying chemical sensitizers of fragrance ingredients
A. Del Bufalo, F. Tourneix, S. Martinozzi-Teissier, F. Cottrez, H. Groux, J. Cotovio

IL-18 secretion as a marker for identification of contact sensitizers in the EpiDerm™ in vitro human skin model
J. Oldach, S. Letasiova, H. Kandarova, M. Klausner, P. J. Hayden

Refinement of the peroxidase peptide reactivity assay (PPRA) and prediction model
J. A. Troutman, C. Goebel, H. J. Dai, R. L. M. Dobson, M. Quijano, G. F. Gerberick

A dermal sensitization assay using SkinEthic™ RHE
G. DeGeorge, M. Troese

A highly differentiated 3d epidermal skin model to characterize skin sensitizers
L. Pratt, M. Troese, G. DeGeorge

Implementation of the RHE / IL-18 sensitization assay – Accuracy, sensitivity and specificity evaluation
E. Andres, J. De Brito, A. Hundt, E. L. Roggen, C. Dini, P. J. Ferret

Co-culture assay for the identification and investigation of dermal sensitizers (epi-dc)
G. DeGeorge, M. Troese, L. Pratt , M. Piehl, P. Hayden, S. Ayehunie

Probabilistic Hazard Assessment for Skin Sensitization Potency using Machine Learning to Design Integrated Testing Strategies
V. Rocha, T. Luechtefeld, A. Maertens, A. Kleensang,, T. Hartung

Session II-13: Endocrine disruption

Performance of the BG1Luc and ER beta-lactamase estrogen receptor transactivation assays in Tox21 compound screening
W. Casey, R. Huang, J-H. Hsieh, K. Shockley, S. Sakamuru, M. Xia, R. Tice, L. Rinckel, P. Ceger, D. Allen

Development of reverse toxicokinetic models to correlate in vitro and in vivo estrogen receptor activity
X. Chang, N. Kleinstreuer, P. Ceger, J-H. Hsieh, J. Hamm, B. Jones, N. Choksi, L. Rinckel, D. Allen, W. Casey

Targeted testing of FXR Tox21 active compounds
J. Hamm, W. Casey, C. Hsu, K. Houck, X. Chang, D. Allen, S. Kullman

An Inter-laboratory validation study of an androgen receptor stably transfected transcriptional activation (AR STTA) assay for a new OECD test guideline
A. Ono, M. Takeyoshi, Y. Maeda, N. Suzuki, H. Kojima, S. Takeuchi, J. H. Hong, H. S. Lee, A. M. Vinggaard, J. Odum, S. Laws, H. Kojima

New biomarkers for endocrine disruption evaluation on microplate using a human placental cell line
A. Wakx, T. Fournier, P. Rat

Estimation of estrogen receptor binding affinity by QSAR approach
M. Rucki, H. Bendová, J. Sosnovcová

Session II-14: Reproductive & developmental toxicology

In vitro cytotoxicity assessment of a trichotecene mycotoxin T-2 toxin on SerW3 cells
E. Karacaoğlu, G. Selmanoğlu

Development of alternative tests for reproductive and developmental toxicity: whole embryo culture in humanized CYP3A (CYP3A-HAC) mice
M. Akita, Y. Kazuki, S. Abe, N. Ishizuka, A. Yokoyama, T. Kamataki, M. Oshimura

Acrylamide and Its Metabolite Glycidamide can Effect Antioxidant System and Steroidogenesis in Leydig Cell in vitro
Y. Tunali, M. Erkan

Effects of Acrylamide and Glycidamide on Antioxidant Systems and Steroidogenic Enzymes on TM4 Sertoli Cell
Y. Tunali

Acrylamide and Glycidamide can Effect Leydig and Sertoli Cell Viability, Oxidative Damage and Apoptosis in vitro
B. Orta-Yilmaz, N. Yildizbayrak, M. Erkan

Development of in vitro detecting method for developmental toxicity using mouse embryonic stem cells - Hand1-Luc Embryonic Stem cell Test
N. Suzuki, F. Le Coz, H. Nagahori, K. Saito

Express assessment of geno-, cyto- and embryotoxic effects of potential teratogens using an integrated in vitro test-system based on rat whole embryo culture
I. Strekalovskiy, L. Shabasheva, V. Popov, Y. Panfyorova

Toward validation of a human in vitro assay for developmental toxicity assessment
L. Egnash, K. Conard, J. Palmer, A. Smith, P. West, R. Burrier, E. Donley, F. Kirchner

Using Cellular Signals to Assess Developmental Toxicity of Compounds in a Mouse Embryonic Stem Cell System
M. A. T. Teunis, S. Verkoeijen, P. Mika, K. Lai, P. T. Theunissen, A. H. Piersma, C. A. M. Krul

New in vitro method to image and quantify neural cell migration
P. Salonen, T.Toimela, J. Kreutzer, P. Kallio, T. Heinonen, A. Price, T. Ylikomi

Session II-15: Genotoxicity/Carcinogenicity

Evaluation of potential carcinogenic effects of SiO2 nanoparticles in human lymphocyte culture using genetic and functional markers
I. Kolesnikova

Genotoxic evaluation of essential oils: comparison between classical and automated micronucleous in vitro assay
N. M. Esteves-Pedro, P. S. Lopes, M. B. Mathor, D. Perez, R. Albuquerque, P. H. Moreno , T. M. Kaneko

Hydra as a small animal model for environmental toxicity testing: standardization of comet assay
A. Murugadas, Md. Zeeshan, P. Balaji, P. C. Vinodha, K. Thamaraiselvi, M. A. Akbarsha

The use of SHE cell transformation assay to predict the transforming potential of non genotoxic carcinogens
A. Colacci, M. Vaccari, M. G. Mascolo, S. Perdichizzi, C. Zanzi, F. Rotondo, C. Parfett, R. Corvi, S. Grilli

A new resource for predictive toxicology: EURL ECVAM genotoxicity and carcinogenicity database of positive Ames test chemicals
R. Corvi, F. Madia, A. Worth, M. Whelan

Reduction of misleading (“False”) positive results in mammalian cell genotoxicity assays and its favourable impact on animal use
P. Fowler, K. Williams, L. Jeffrey, J. Young, N.J. Hewitt, R. Fautz, D. Kirkland, P. Carmichael, P. Pfuhler

Development of a method to compare genotoxic hazard of water samples
A. R. Van Rompay, B. Martijn, S. Verstraelen, E. J. M Penders, P. K. Baggelaar

In vitro cytotoxicity and genotoxicity testing using upcyte hepatocytes
A. Noerenberg, V. Sonntag-Buck, N.J. Hewitt, S. Heinz, J. Braspenning

In vitro immunotoxicity screening of xenobiotics using human lymphoblastoid cell lines
T. Markovic, M. Gobec, D. Gurwitz, I. Mlinarič-Raščan

Investigation of the genotoxic potential of a new Brazilian botanical extract – a case study
C. Zacarias, A. Armelini, K. Lucena, K. Arroteia, P. Araujo, K. Assanome, D. Pedroso

RSMN (Reconstructed Skin Micronucleus Assay): Update on the ongoing validation
S. Pfuhler, M. Aardema, B. Barnett, E. Costin, S. Hoffmann, G. Mun, G. Ouedraogo, S. Roy, R. Curren

The Automated FADU-Assay to Quantify Formation and Repair of DNA Strand Breaks – A Rapid and Meaningful Genotoxicity Quantification
M. Pilartz, M. Unger, M. Moreno-Villanueva, I. Pfitzner

Session II-16: Inhalation toxicology

Prototype inhalation risk assessment for biopersistents using adverse outcome pathway (AOP) approaches
P. Jones, B. Bradford, P. Fowler, A. Furniss, A. Homan, R. Stark, A White

The use of a Long Shelf-life 3D Model of the Human Airway Epithelium (MucilAir™) for acute and repeated dose Respiratory toxicity assessment
S. Constant, S. Huang, L. Wiszniewski

A technical approach on precise determination of minimal mass of deposited airborne particles
A. Schafmeister, A. Geisszkopf

Fundamental improvements of the air-liquid interface technique for use in in-vitro inhalation toxicology
J. Knebel, D. Ritter, C. Brodbeck

Applicability of rat precision-cut lung slices in evaluating nanomaterial cytotoxicity and inflammation
U. G. Sauer, S. Vogel, A. Aumann, A. Hess, S. N. Kolle, L. Ma-Hock, W. Wohlleben, M. Dammann, V. Strauss, S. Treumann, S. Gröters, K. Wiench, B. van Ravenzwaay, R. Landsiedel

In vitro assessment of airway effects of key products from ozone-initiated oxidation of limonene and printer exhaust
I. Nelissen, F. Maes, A. Jacobs, S. Remy, S. Verstraelen, E. Goelen

The CULTEX® RFS Compact: the next generation of CULTEX® systems for the direct exposure of cells at the air-liquid interface
N. Moehle

Session II-17: Ecotoxicology

Using chlorella kessleri for monitoring of water quality
V. Harvenda, L. Umar, J.Wiest

Strategies to reduce the number of fish used in ecotoxicity tests
G. Stoddart, K. Sullivan

Revisiting Hydra in the wake of vision Tox21
M. Zeeshan, A. Murugadas, M. V. Muhsin, B. R. Ramaswamy, M. A. Akbarsha

Novel in-vitro tests for analysis of indoor environment toxicity correlated to human health
M. Salkinoja-Salonen, M. Gasik, M. A. Andersson, J. Atosuo, A. Hyvärinen, K. Huttunen


Theme III – 3Rs in Academia & Education

Session III-1: 3Rs in academic education, training programs and anticipated needs

Survey of Canadian research and teaching institutions on strategies for implementation of the Three Rs
A. Plante, J. Alcorn

Experience in the use of alternatives to animal experimentation in the learning capability of undergraduate students of human physilology at the Universidad de Guadalajara, Centro Universitario de la Costa 2008 to 2014
V. Rojas-Sahagún, S. Viruete-Cisneros, R. Preciado-González, M. Greig, K. Barrios-Pérez, L. Villaseñor-Loeza, J. Esparza-Barajas, A. Delgado-Romero, P. Santiago-Teodoro, N. Mera-Riofrio

An Indian educational initiative to promote the use of alternatives to animal testing with special reference to 3D reconstructed human tissues
A. Riyasdeen, C. Pellevoisin, A. Alonso, M. A. Akbarsha

Continuous education and trainings promotes the 3Rs alternative methods development in China
S. Cheng

Conscientious objection to harmful animal use within veterinary and other biomedical education
A. Knight

An inspiring book telling the personal stories on the Replacement, Reduction and Refinement of laboratory animal use
M. Schilders-van Boxel, I. Toussaint

EPA’s Toxicity Forecaster Research Effort Communications and Outreach Overview
M. Linnenbrink

4-year experience teaching alternative methods to animal use in toxicology
F. Caloni

Graduate training program BB3R
V. Kral, G. Weindl, J. Plendl, H. Fink, B. Kleuser, A. Pries, R. Preissner, A. Luch, G. Schönfelder , R. Lauster, M. Weber, M. Schäfer-Korting

Experiments on Animals: Ideals of Humane Education & Real Practice
I. Merkulova, T. Mishatkina, T. Hlinkina

Нumane alternatives application on biology school lessons for the purposes of eco-ethical thinking formation among youth
L. M. Loginovskaya

Humanising and modernising medical education: Change in the Ukraine
I. Strelchenko , D. Leporsky, N. Jukes

Session III-2: Funding Agencies and Funding Programs

The Danish 3R-Centre
L. B. Broenden , T. Bengtsen, C. L. Nellemann

Motivating synthesis of evidence part of more knowledge with fewer animals programme
E. van Oort, C. Schuttevaer, R. Diemel , U. Gangaram Panday

Session III-3: Innovative teaching and training tools

NORINA: information on animal alternatives in a changing world
K. Smith

Development of the New Korean Teaching Aids on the Three Rs Principles: Translation of “The Three Rs and the Humanity Criterion”
B. I. Choe, G. H. Lee, B. H. Lee, S. Trigwell, M. Balls

Animal alternatives in veterinary teaching- status and scope in an Indian veterinary university
A. B. Jacob, C. Leena, M. R. Saseendranath

Peer mentoring and alternatives to using animals as a strategy for education in the life sciences
A. Meneses-Parga, S. Viruete-Cisneros, Y. De La luz-Esparza, D. López-Grano, P. Gay-Hernández, I. Casillas-Martínez, O. López-Padilla, E. Díaz-Pelayo, M. Martínez-Toscano, R. Preciado-González

Overview of the free virtual experiments - computer simulations for teaching Pharmacology
D. Modun, E. MacDonald

Emphasis on Zoology curriculum based on non-invasive teaching methods of Physiology and Anatomy has significantly reduced the animals use in practical classrooms in India
K. K. Sharma, V. Sharma, N. Sharma, M. A. Akbarsha

Digitization of zoology museums can reduce the number of wild animals sacrificed for teaching of biosystematics: Another dimension to implementation of 3Rs principle in teaching
V. Sharma, K. K. Sharma, N. Sharma, M. A. Akbarsha

Prokaryotes can be used as an effective animal alternative in genotoxicity testing of antifertility and xenobiotic compounds in routine lab courses
N. Sharma, V. Sharma, K. K. Sharma, M. A. Akbarsha

The development of a clinical skills laboratory at Ross University School of Veterinary Medicine
B. Grevemeyer, A. Knight

 ‘Live zoology’ an effective, non-invasive replacement alternative to animal dissections in zoology curriculum
M. C. Sathyanarayana, M. A. Akbarsha

Virtual Physiology: Computer Laboratories for Life Science Education
A. Tchaptchet, J. R. dell‘Oro-Friedl, D. Immer, H. Schneider, T. Braun, H. A. Braun

3Ts teaching touching toxicology in veterinary medicine degree: a project
F. Caloni, L. Müller, C. Cortinovis, M. Balogh

Synthesis of evidence and systematic reviews of animal studies: urgently needed innovations in laboratory animal science education
M. Leenaars, M. Ritskes-Hoitinga

Humane education strategies for veterinary education and training in colombia
M. Chaparro, A. Guerrero, V. Arcila, V. Quintero

Three databases for alternatives in education and training
N. Jukes, V. Danko, D. Leporsky

Session III-5: Sharing best practices in LAS education and training

Students’ perception of e-learning in Laboratory Animal Science training
A. Costa, A. Costa, I. A. Olsson


Theme IV – Communication, Dissemination and Data Sharing

Session IV-2: Scientific reporting standards (in vivo and in vitro)

Study on the scientific validity of animal experiments in Switzerland
T. S. Reichlin, L. Vogt, H. Würbel

Session IV-3: Retrospective analysis / non-technical summaries (2010/63)

Public openness in laboratory research: a survey study
E. Kwok, E. H. Ormandy, D. M. Weary

Session IV-4: Information systems and databases

The Status of a New Database Construction for the Safety Results of Korean Alternative Researches
K.-H. Park , B.-H. Kim

Monitoring and evaluation of developments in animal use and 3R-alternatives
S. Deleu, M. van Zijverden

Use of ASPCA Animal Poison Control Center’s Toxicology Database for Characterizing Toxicity of Human Medications in Pets
S. Khan, M. McLean

3R Guide: a new database of 3R resources
A. Smith, T. Allen, Ø. Wærenskjold

The EURL ECVAM Search Guide – promoting global standards for searching 3Rs information
A. J. Roi, B. Grune, Jon Richmond

An optimized hypothermic storage medium capable to maintain histology and functions of skin tissue and allow epidermal stem cell isolation
C. Chesné, N. Rougier, M.Kermorvan


Theme V – Efficacy and Safety Testing of Drugs and Biologicals

Session V-1: Pathways based assays in drug development

Cardiotoxicity of captopril in chick embryos
Y. Yoshiyama, T. Tamaki, T. Kozuki, M. Okayasu, T. Miyazaki, M. Kawakami, H. Ariumi

A zebrafish bioassay to evaluate gastrointestinal function
T. B. Cole, S. C. Cassar, J. J. Lynch, S. L. Koeniger, K. Hempel, W. R. Buck, H. Yang, H. Lorenz, B. Tornesi

Inhibitory effects of ambroxol on type A seasonal influenza virus infection in vitro
T. Tamaki, H. Ariumi, Y. Yoshiyama

Electron Paramagnetic Resonance (EPR) Spin Labeling Study of HT-29 Colon Adenocarcinoma Cells after Hypericin-Mediated Photodynamic Therapy
A. Kılıç Süloğlu, D. Yonar, M. M. Sünnetçioğlu, G. Selmanoğlu

Nerve-muscle-cultures as an in vitro tool for pharmacologic testing
V. Eckle, C. Grasshoff, B. Antkowiak

Use of upcyte® human hepatocytes to CYP inhibition and induction screening
S. D. Ramachandran, N. J. Hewitt, R.Eudenbach, S. Heinz, B. Muenst, J. Braspenning

Trypanosoma cruzi: in silico and in vitro alternatives for identifying new antiparasites
S. C. Bourguignon , H. P. Araújo, G. C. Lechuga, S. B. Nascimento, A. L. Lourenço, V. F. Ferreira, H. C. Castro

Antithrombotic potential of synthetic 1,2,3-triazoles evaluated through in vitro and in silico approaches
L. Moura, A. de Almeida, A. da Silva, S. Ferreira, V. Ferreira, A. Fuly

Session V-2: Disease models in vivo

Comparative assessment of cytotoxic effects of garlic oil and 2,2'-dithio-bis(N,N-diethyl)ethanamine on human umbilical vein endothelial cells in culture
D. Prokofeva, N. Goncharov

Trends in the use of genetically modified mice and their efficacy as a model for human disease
K. Coll, A. Bennett

Diet-induced iron deficiency anemia in rats – in vivo model
J. Reguła, J. Suliburska, E. Król, Z. Krejpcio

Role of drug transporters in pancreatic adenocarcinoma therapy
M. Ehrlichova, M. Kocik, B. Mohelnikova-Duchonova, V. Brynychova, K. Elsnerova, V. Hlavac , M. Oliverius, E. Honsova, J. Mazanec, J. Hlavsa, Z. Kala, P. Soucek

Session V-3: Potency testing of human and veterinary vaccines

Thermal imaging - alternative concepts for refinement and reduction of animal use for testing of biologicals
A. Hoffmann, C. Dumke, F. Mohs, E. Balks, J. Hermans, M. Bernau, P. Kremer, M. Toda, U. Salge-Barthels, K. Cussler

An Animal-free Batch Testing Method for Alum-Adjuvanted Veterinary Rabies Vaccines
V. Jakel, K. Duchow, B. Kraemer, M. Bastian

Notes on the validation of Vero cell test for the absence of residual diphtheria toxin
F. A. Freitas, J. Kapronezai, W. Quintilio

A serological assay to determine the potency of inactivated rabies vaccines for human use
B. Kraemer, H. Schildger, H. Wegner, V. Euteneier, J. Klimek, K. Duchow

In vitro characterization of adjuvant biological activity and safety
S. Richard, M. J. Diana, P. Probeck, J. Haensler, A. Saulnier, N. Nougarede

Development of relevant serological methods for replacing in vivo seroneutralization tests used for determining Potency of Diphtheria, Tetanus and whole-cell Pertussis vaccines
M. L. Chovel, A. Mandiarote, N. Gutiérrez, L. Herrera, J. F. Núñez, O. Valle, I. Ontivero, T. Mahy

Study on the establishment of Standard material for pertussis toxin and Standard pertussis strain
J.-I. Ahn , H. Oh, K. Kim , H. Song , H. Kim, S. Lee, M.-Y. Bae, J. Park, S. Kim, J.-G. Kim

Determination of Effectiveness of Immunized Chickens’s Immuneglobuline Y against Salmonella Infection in Mice
H. Hadimli, Z. Sayin, A. Sakmanoglu, G. Sanioglu

Study of an Alternative pyrogen test for blood product
J.-H. Kim, J.-Y. Lee, D.-H. Ryu, Y.-S. Kang, W.-H. Lee, H.-O. Kim, H.-K. Oh, Y.-L. Kim, K.-T. Nam, C.-Y. Ahn

Potency evaluation of Bothrops snake venom: a cytotoxicity assay as an alternative to the murine model
H. Araujo, D. F. B. Cavalcanti, A. L. Fuly , S. C. Bourguignon

Evaluation of serological test for whole cell pertussis DTP vaccine
J. Kapronezai, F.A. Freitas, W. Quintilio

Optimisation of an in vitro assay system as an alternative to current murine histamine sensitization test for acellular pertussis vaccines
K. Markey, C.T. Yuen, C. Asokanathan, A. Douglas-Bardsley , D. Xing

Development of a common immunogenicity assay on guinea pigs for the potency testing of multicomponent vaccines
M. Chabaud-Riou, F. Guinchard, C. Lyonnais, A. Lefebvre, S. Guichard, S. Perrin, S. Imbert, S. Uhlrich, D. Gaillac, D. Petre

Avian encephalomyelitis live vaccines - alternative methods for batch testing
L. Rimpel, S. Haege, D. Loos, C. Jungbaeck, A. Motitschke

Refinement and reduction of 3R; in vivo imaging method can refine experimental procedures with less number of animals
K.-J. Hong, P.-G. Park, Y.-H. Kim, Y.-W. Shin, G.-E. Rhie, H. Youn, S. Y. Han

The BINACLE (binding and cleavage) assay allows in vitro detection of active tetanus neurotoxin in vaccines
H. A. Behrensdorf-Nicol, U. Bonifas, K. Weißer, B. Krämer

Session V-4: Medical devices and Biologicals

Magnetic resonance imaging – a way to reduce animal numbers for safety testing of veterinary medicines
M. Bernau, P.V. Kremer, L.S. Kreuzer, E. Pappenberger , K. Cussler, A. Hoffmann, D. Emrich, M. Leipig, W. Hermanns, A.M. Scholz

Pulmonary artery hemostasis: a model for surgical stapling and sealing
C. Henderson

In-vitro potency determination of botulinum neurotoxins A and B
E. Wild, H. A. Behrendsorf-Nicol, U. Bonifas, B. Kegel, B. Kraemer

Generation of neurons derived from stem cells and cell lines to develop cell based replacement assays for botulinum toxins and antitoxins
N. Bak, B. Englich, C. Rasetti-Escargueil, D. Sesardic

Pred-Immune; A novel integrative statistical approach for the prediction of the risk of immunogenicity of biologics
G. J. D. van Mierlo, R. H. Klein Entink, M.-L. H. M. H. de Zeeuw-Brouwer, F. J. Tielen, C. A. M. Krul, B. O. Fabriek

Photographic evaluation of skin lesions in batch potency testing of tuberculins – a reduction approach
E. Balks, C. Dumke, F. Mohs, K. Cussler, E. Werner, A. Hoffmann

Local lymph node assay on five medical devices including dental materials for alternative safety evaluation
I. L. Ahn, S. Y. Park, J. Y. Yang, S. Y. Kim, J. S. Lee, J. H. Lee, T. S. Kim, K. H. Choi

Towards a full-function molecular-based assay for Botulinum Neurotoxin
G. B. Stevens, T. Binz, W. Römer, T. van Oordt, G. A. Urban, R. Zengerle, F. von Stetten

An alternative micromethod to assess the procoagulant activity of Bothrops jararaca venom and the efficacy of antivenom
N. Oguiura, J. Kapronezai, T. Ribeiro, M.M.T.Rocha, C.R.Medeiros, J.R.Marcelino, B.C.Prezoto

Development of tridimensional murine and primary human osteoblastic cells models for biocompatibility studies
L. Restle, D. Costa, E, Lourenço, R. Bachinski, A. Linhares, G. Alves

The application of rat hepatocyte aggregate for cytotoxity assay
M. Shinohara, K. Komori, T. Fujii, Y. Sakai

Development of a Mock Circulatory System for Evaluation of Mechanical Heart Valves
M. Ross, S. Gregory, G. Tansley, M. Pearcy

Applicability of Monocyte Activation Test (MAT) in the routine of the quality control laboratory
C. Caldeira, O. Presgrave, A. Moraes, I. Delgado

Monocyte Activation Test (MAT): identification of monographs that recommend Rabbit Pyrogen Test (RPT) and Bacterial Endotoxin Test (BET) as a kick-off for showing applicability of MAT
C. Caldeira, M. Cruz, J Freitas, O. Presgrave, A. Moraes, I. Delgado

Assessment of pyrogenic contamination with Lipoteichoic Acid (LTA) in the Monocyte Activation Test (MAT) and Rabbit Pyrogen Test (RPT)
I. Gimenes, C. Caldeira, O. Presgrave, M. Villas-Boas

Isolation of Shiga Toxin-Producing Escherichia coli (STEC) Using Magnetic Beads
J. H. Kim, S. J. Lee, K. W. Seo, S. Y. Han, Y. H. Kang, G. T. Chung


Theme VI – Human Relevance

Session VI-1: In vitro disease models

Evaluation of in vitro inhibitory effect of Enoxacin on Babesia and Theileria parasites
M. A. Omar, M. A. Rizk, S. M. Elsayeda, A. ElSify, A. A. Salama, S. S. AbdElgayed, N. Yokoyama, I. Igarashi

Inter-laboratory validation of an innovative huFcERIa-RBL-2H3 degranulation assay for in vitro allergenicity assessment of whey hydrolysates
K. Knipping, M. van Roest, L. Kruijssen, M. Smits, L. Cox, N. de Jong, P. Simons, R. Teshima, L. Knippels, R. Pieters

An assessment of the cell stress response of lung epithelial cells exposed to cigarette smoke aqueous extracts
M. Taylor, G. Moore, T. Carr, N. Cockcroft, F. Lowe, G. Phillips

In vitro models for neuroimmunological diseases
S. M. Burm, E. A. Zuiderwijk-Sick, J. Veth, C. van der Putten, A. E. 't Jong, P. M. Weert, J. J. Bajramovic

Simulating the first phase of bone fracture healing in vitro – Possible or not?
A. Lang, P. Hoff, J. Neuhaus, I. Ponomarev, D. Barnewitz, F. Buttgereit, T. Gaber

A model of neuronal hyperreactivity in passive sensitized human organotypic tissue
S. Schindler, S. M. Jimenez-Delgado , A. Braun, K. Sewald

Human organotypic cancer model
S. Konzok, S. Schindler, A. Braun, K. Sewald

Nucleus pulpous and annulus fibrosus cell isolation from human intervertebral disc
L. Gradisnik, T. Velnar

Validation of a mock circulation loop for the development of ventricular assist devices with physiological data
J. Fröhlich, J. P. Pauls, S. D. Gregory

Low concentrations of cigarette smoke extract act cytotoxic on human alveolar epithelial cells type II
S. Seehase, B. Baron-Luehr, C. Kugler, E. Vollmer, T. Goldmann

Chorioallantoic membranes as a living support for skin and human tumor cells xenografts
H. Dabboue, G. Marti-Mestres , J-P. Molès, L. Meunier, X. Rebillard, P. Nirdé

Application of in vitro skin models for cosmetic product efficacy: UV protection, skin lightening, skin hydration and anti-aging
M. Bachelor, A. Armento, B. Breyfogle, G. Stolper, J. Oldach, M. Klausner, P. J. Hayden

Session VI-2: Use of stem cells in screening

Genome-wide gene expression analysis by RNA-seq in murine embryonic stem cells
H. Tani, J. Takeshita, H. Aoki, M. Gamo, M. Torimura

Establishing an in vitro screening test for developmental neurotoxicity
J. Huang, S. Cheng

Human in vitro adipogenesis assay for testing modulators of adipogenesis
O. Huttala, JR. Sarkanen, T. Ylikomi, T. Heinonen

Profiling of drugs and environmental chemicals for functional impairment of neural crest migration in a novel stem cell‑based test battery
G. Pallocca, B. Zimmer, N. Dreser, T. Waldmann, K.H. Krause, C. van Thriel , J. Hengstler, A.Sachinidis, S. Bosgra, M. Leist

Session VI-3: Human biomarkers

Funding innovation to foster decreases in compound attrition and the 3Rs: a new process
G. Dal Negro

Proton magnetic resonance spectroscopy and recursive partitioning analysis of brain metabolism data from an Alzheimer’s disease cohort offers a replacement alternative for animal studies on disease prediction
A. C. Londono, F. X. Castellanos, A. Arbelaez, A. Ruiz, D. C. Aguirre-Acevedo, A. M. Richardson, S. Easteal, B. A. Lidbury, M. Arcos-Burgos, F. Lopera

From transient transcriptome responses to disturbed neurodevelopment: divergent response patterns and epigenetic modifications triggered by the same drug
S. Klima, N. V. Balmer, E. Rempel, A. Sachinidis, J. G. Hengstler, J. Rahnenführer, T. Waldmann, M. Leist

Waves of gene expression as basis to define windows of sensitivity for developmental neurotoxicity
N. Dreser, N. Balmer, S. Klima, T. Waldmann, M. Leist

Session VI-4: Absorption, distribution, methabolism and excretion (ADME)

Porcine buccal mucosa as an in vitro route model for systemic drug delivery evaluation
M. Sacha, H. Haltner

Biotransformation of testosterone and 2,4-toluenediamine by human skin and reconstructed tissues
L. Grohmann, W. Klipper, M. Schäfer-Korting, G. Weindl

Characterization of monoamine oxidases, steroid-5α-reductases, sulfotransferases and glutathione S-transferases in reconstructed skin tissues and human skin ex vivo
L. Grohmann, M. Schäfer-Korting, G. Weindl

Influence of receptor fluid on dermal absorption of caffeine
K. Trenz, S. Bruszies, H. Hofman-Huether

Microdosing of New Biological Entities (NBEs) in healthy volunteers: a safe and fast tool to predict clinical pharmacokinetics
M. L. H. Vlaming, E. van Duijn, R. Brands, A. D. Windhorst, N.H. Hendrikse, S. Bosgra, M. C. de Koning, W. Seinen, P. A. M. Peeters, W. H. J. Vaes

A comparison of xenobiotic metabolism enzyme profiles in human skin and in vitro skin models
N. J. Hewitt, F. Fautz, P. Fowler, J. Kenny, A. Latil, G. Quedraogo, K. Reisinger, M. Klaric, P. Pfuhler

Prediction of the brain distribution of drugs in human using in vitro human blood-brain barrier model derived from stem cells
R. Cecchelli, D. Hallier-Vanuxeem, S. Aday, M. Culot, E. Sevin, C. Almeida, M.-P. Dehouck, L. Ferreira

Session VI-5: Epithelial biobarriers

Establishment of an intestinal model for oral vaccination testing
C. Lotz, M. Schweinlin, H. Walles, M. Metzger

Full-thickness tissue engineered development using human keratinocyte and adipose tissue derived mesenchymal stem cells
R. C. Mosca, D. Steffens, C. Pelissari, A. Mantesso, M. B. Mathor

HuIFN-αN3 and 10% PBS Holocene grain wash-out affect the Glutathione and lipid peroxidation in CaCo-2 cells
B. Filipic, L. Gradišnik, S. Koren, E. Šooš, H. Mazija

Evaluating reconstructed cutaneous squamous cell carcinoma
C. Zoschke, H. F. Merk, S. Küchler, G. Weindl, M. Schäfer-Korting

Evaluation of serum growth factors in wound healing using a full-thickness in vitro human skin model
M. Bachelor, A. Armento, G. Stolper, J. Oldach, M. Li, M. Klausner, P.J. Hayden

Use of reconstructed human epidermis as alternative models for efficiency studies against the penetration of organophosphates
C. Bignon, D. Josse, S. Amigoni, E. Taffin de Givenchy, J. Pelletier, S. Briançon, F. Guittard


Theme VII – Ethics

Session VII-2: Ethics of using animals

Virtue ethics: an ethical approach for non-clinical drug development?
M. Spatzenegger

A patient tail
J. Johnson, C. McLeod

Animal rights and ethics in research: Is it possible a non-speciest approach?
J. J. Rocha

A system for retrospective assessment of cumulative severity to identify targeted refinements
S. Wolfensohn, M. Dennis, S. Sharpe

Openness and accountability of animal research: a focus group study with local stakeholders at a Canadian university
E. Ormandy

Trends in animal use at US research facilities
J. R. Goodman, A. Chandna, K. V. Roe, S. H. Suiter

1R is the new 3Rs
C. Redmond

The curious language of animal research and how it jeopardizes the public trust
F. McMillan

Life after research: the psychological well-being of animals following release from the laboratory
F. McMillan

Animal experimentation: transparency and public concerns
K. Herrmann

Emerging Trends in Alternative to Animal Experimentations
N. Verma

Attitudes on animal use shown by scientists at workshops in Taiwan
D. Fry, M. Jennings, P. Littlefair

The harm-benefit analysis within the evaluation of animal experimentation projects. How to assess criteria like species, number of animals or death of animals?
V. Marashi, N. Alzmann, H. Grimm

Using minipigs in pre-clinical studies in Russia
A. Rybakova, A. Selezneva

Session VII-3: Ethical evaluation

Environmental Impacts of Animal Research and Testing
K. Groff, T. Capaldo

Analysis of animal experimentation oversight committee membership at US institutions
A. Chandna, L. A. Hansen, J. R. Goodman

Great ape disgrace: how the US laboratory oversight system failed chimpanzees
J. R. Goodman, A. Chandna

Germany: Animal welfare representative legal action and its effects on animal experimenting
C. Baumgartl-Simons, C. Hohensee, C. Ledermann

German animal welfare act in breach with Directive 2010/63/EU
I. Ruhdel, K. Wagner, R. Kolar

The “benefits” standard: how can it better protect animals?
M. Matevia, A. Rowan

Session VII-4: Distress evaluation

Norecopa: using a national consensus-platform to promote the 3Rs
A. Smith

Emotional pain: why it can matter more to animals than physical pain
F. McMillan

The complexity of animal well-being: advances in our understanding of quality of life, welfare, and happiness – and why it matters in animal research
F. McMillan

Session VII-5: Benefit evaluation

Benefit evaluation within project evaluation under directive 2010/63/EU
D. Anderson, S. Louhimies


Theme VIII – Refinement and Welfare

Session VIII-1: Non-human primate use and welfare

European Primate Network EUPRIM-Net II: Advancing 3Rs and international standards in biological and biomedical research
V. Stephan, B. Pauling, R. Teepe, S. Treue

A cage-based behavioural testing system for NHP – Towards a cognitive neuroscience without restrained animals
M. Niessing, M. Berger, L. Burchard, A. Calapai, K. Heisig, V. Stephan, S. Treue, A. Gail

Using an automated system of positive reinforcement training to refine the process of behavioural training and reduce the need for food control in rhesus macaques
J. Tulip, J. B. Zimmermann, F. de Carvalho, D. Farningham, A. Jackson

Optimisation of preclinical use of Non-human primates (NHP) by species-specific Monocyte activation test (MAT)
J. Hermanns, C. Bache, E. Holznagel, B. Becker, J. Kaiser, I. Spreitzer

Session VIII-2: Best practice welfare approaches – Mouse

Microenvironment in reusable and disposable individually ventilated cages: a comparative study
L. Contalbrigo, G. Milite

Husbandry and enrichment for the recently protected common European cuttlefish (Sepia officinalis)
G. M Cooke, B. M. Tonkins

Refinement and efficiency through serial microsampling – cost/benefit analysis
N. Bratcher, P. Ebert, W. Buck, L. Medina

Vaccination in lambs – An animal welfare issue?
M. Bernau, P. V. Kremer, L. S. Kreuzer, S. Schwanitz, K. Cussler, A. Hoffmann, M. Leipig, W. Hermanny, A. M. Scholz

Refining oral gavage: assessing and improving welfare in the laboratory-housed dog using a Welfare Assessment Framework
L. Hall, S. Robinson, H.M. Buchanan-Smith

Refinement in pain relief in sheep fetal surgery
H.-M. Voipio, E. Heikkinen, S. Laaksonen, L. Haapala, A. T. Heikkinen, J. Räsänen, T. Erkinaro, G. Acharya, M. Kokki, H. Kokki

Putting animal welfare principles and 3Rs into action
K. Blumer

Severity classification in German animal research applications
K. Herrmann, P. A. Flecknell

Refinement in German animal research applications
K. Herrmann, P. A. Flecknell

Determination of the optimal dose of benzocaine hydrochloride in euthanasia of frogs (Rana catesbeiana)
A. Pereira, M. A. Lallo

Cognition studies refined with pet animals
M. Norring, S. Somppi, H. Törnqvist, L. Hänninen, O. Vainio

Assessing physiological and behavioral responses over time in laboratory beagles
L. Dietz, C. Bol, F. Ohl, A. Ortolani

Refinement strategies to improve the survival rate in mouse models for influenza A virus infections
M. Boldrin, A. Romero Tejeda, G. Beffagna, R. Aiello, I. Capua, C. Terregino, G. Cattoli, F. Mutinelli

Update on best practice approaches to the welfare and husbandry of fish, cephalopods and decapods
A. Smith, P. Hawkins, T. Kristiansen, C. Mejdell

Use of Eclipta prostrata extract prevents tissue damage induced by snake venoms immunization in horses during antiofidic serum production
M. A. Strauch, L. E. R. Cunha, P. N. C. Souza, L. R. Meirelles, P. A. Melo

Decreased use of experimental animals through optimization of experimental design for efficacy studies
R. Stoop, A. M. Zuurmond, J. R. O. Hanemaaijer, S. van Buuren, P. L. P. Van Loo

Stress response to blood sampling from dorsal pedal vein compared to saphenous vein in mice
S. Ledahl, N. Delaleu, P.Vogelsang

Health Monitoring of rodents in microisolation cages – A rationale approach (with an eye to the 3Rs)
M. Skowron

Laboratory mouse euthanasia: aversion and refinement
C. Moody, I. J. Makowska, D. M. Weary

Session VIII-3: Humane principles in experimental techniques and benefits of 3Rs

Principles of animal modelling in psychiatric research with respect to 3R rules
H. Tejkalová

Gavage incidents- a urgent need for action
K. Taylor, L. Rego

The development of hormone loaded diets to promote xenograft growth
A. Ritchie, P. Collier, N. Nicolaou, A. Grabowska

Humane principles of using animals in Russia
V. Makarov, M. Makarova, A. Selezneva, A. Rybakova

Session VIII-4: Avoidance of severe suffering

Promising in vitro alternatives for nutrition studies in cattle
A. B. Jacob, V. Balakrishnan

A road map towards ending severe suffering
E. Lilley, P. Hawkins, M. Jennings

Session VIII-5: Culture of care

Changing the culture of laboratory rat care
I. J. Makowska, J. Sato-Reinhold, D. M. Weary


Theme IX – Global Cooperation, Regulatory Acceptance and Standardization

Session IX-1: Activity updates from international scientific societies

The International Society for In Vitro Methods (INVITROM)
J. van der Valk, R. Cortvrindt, S. Horbach, T. Vanhaecke, H. Witters

'GUTS in the LAB’ – Award for courageous scientists doing excellent 3R research
M. Kuil, M. F. Schilders-van Boxel

Science and awareness: a journey inside the 3Rs – Didactic exhibition project
V. Romanelli, P. Costa, P. Granata, A. Stammati, F.Zucco, F. Caloni, F. Fassio, I. Ferri, M. Kuan, M. T. Iela, A. Vitale, I. De Angelis

COLAMA2012 – the first Latin American congress on alternatives
G. Alves, R. Bachinski, C, Caldeira, L.B. Ballotin, O. Presgrave, J.M. Granjeiro

Session IX-2: Animal welfare implementation across the world

3Rs Dissemination in Asia: Zebrafish embryo toxicity test transferred in Sri Lanka’s universities
F. Busquet

Animal welfare implementation in Switzerland: A successful stakeholder approach based on a joint Animal Welfare Charter
C. Lorber, T. Schnitzer, K. Blumer, J. Coenen

5 Years InVitroJobs – Internet Platform, Job Board and Working Group Network for animal-free research
C. Hohensee, C. Ledermann, C. Baumgartl-Simons, S. Elsner

Use of Animals in Military Medical Training by NATO Nations
J. R. Goodman, S. G. Gala, M. J. Balsam, M. P. Murphy

A new guideline about euthanasia to laboratory animals in Brazil
A. Pereira, M. A. Lallo

Legislation and implementation of alternative methods and outcomes
T. Titma

The transposition of directive 2010/63/EU on the protection of animals used for scientific purposes – a comparison between France, Germany, the United Kingdom and Austria
J. Ricken

3R work structured according to Lean Sigma results in increased refinement and reduction in toxicity testing in the pharmaceutical industry
C. Vult von Steyern, H. Hellmold, I. Cotgreave, B. Platzack, M. Öberg, E. Törnqvist

Alternatives in South Africa: Initiatives in education, research and testing
E. Vercuiel, N. Jukes, L. Sentle, T. Lotter

India takes a giant step forward to protect dogs from testing, with more emerging evidence that dogs add no additional value in toxicity testing
S. Tettamanti Pereira, M. Thew, J. Bailey

Session IX-3: Activity updates from international validation centres

The Brazilian Center for Validation of Alternative Methods (BraCVAM) and the National Network on Alternative Methods (RENAMA): the first years of activities
O. Presgrave, W. Moura, C. Caldeira, L. Balottin, I. Delgado, J. Granjeiro, E. Santos, M. Villas-Boas

In Vitro Laboratory GLP-Certified in Brazil: a successful public-private partnership to achieve international harmonization
F. Medeiros, T. Franco da Cunha, O. Zazuco Higa

Session IX-5: Regulatory acceptance of alternatives

Read across strategy for the assessment of dyes with the aid of a new QSAR system
V. Alberti, C. Rovida, V. Consonni, M. Locatelli, R. Todeschini, M. Kahlberg

SLiM: a smart way from innovations to humans
C. Krul, D.Kegler, L.M'Rabat, R. Pieters, J. Raaijmakers, M-J Schiffelers, M.Teunis, J. van der Valk, R. Vandebriel, R. Woutersen

Developing Regulatory Acceptable In Vitro Methods
C. S. Roper, D. A. Murray, J. Welch, S. Madden

On cosmetics animal testing and be Cruelty free campaign in China
Y. Zhang

Good In Vitro Method Practice (GIVIMP): Guidance on the implementation of in vitro methods within a GLP environment to support regulatory human safety assessment of chemicals
S. Coecke

Considerations for Alternatives to Non-human Primates in Preclinical Safety Assessments
B. Li, X. Geng

Session IX-6: Breaking down barriers and promoting international cooperation on 3Rs

Science Communication in Alternatives to Animal testing: Raising awareness beyond the community
F. Busquet

Regulatory acceptance and use of 3R models for pharmaceuticals and chemicals: Expert opinions on the state of affairs and the way forward
M.-J. Schiffelers

A decision tree to facilitate the replacement of laboratory animals in Brazil
V. C. Bones, C. F. M. Molento

Ban safety tests on animals
M. Kuil

Animal use and cost estimates for the proposed US policy on cosmetics – Comparison with EU and other World States
J. Knight, C. Rovida

Cost Comparison between the Mouse Inoculation Test (MIT) and the Virus Isolation in Cell Culture (VICC) for rabies diagnosis
V. C. Bones, A. H. Gameiro, J. G. Castilho, C. F. M. Molento

Combining 3R strengths in the international arena: together we are strong!
S. Deleu

Working to reduce the use of animals in scientific research – a national approach
W. Reynolds, H. Bodmer, J. MacArthur Clark, K. Dunsford, P. Earl, D. Jones, R. Mueller, V. Robinson, L. Godden

Cooperative industry activities to support international advancement of non-animal testing methods
E. Hill, Q. Zhang, R. Curren

Session IX-7: Harmonising ways to capture pathway-knowledge in toxicology

Quality assurance of emerging technologies in toxicology
M. Bouhifd, T. Hartung


Theme X – Additional sessions

Session X-2: Cosmetics around the world

The LUSH non-animal testing policy
H. Jones

Session X-3: Special lectures

Björn Ekwall memorial foundation
A. Kolman, H. Tähti


Last update: 22 August 2014